CSU
Department of Microbiology, Immunology and Pathology Colorado State University

MIP Molecular Madness

pUC19 2686 bp
Clone It!
Cut plasmids with restriction enzymes, pick your fragment, and ligate it into the vector backbone.
🧬
Antisense
Type the 5'→3' reverse complement before the clock runs out. 4 phases of increasing difficulty.
Antibody Attack
Neutralize incoming pathogens by matching your antibody to the right epitope before they reach the host.
Ribosome Rush
Drag tRNAs onto scrolling mRNA codons to translate a protein — before they scroll past!
10⁰ 10⁻² 10⁻⁴
Serial Simulator
Dilute a bacterial sample through a series of tubes, then plate 1 mL to get a countable result.
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Micro ID
Identify pathogens from images and clinical clues — bacteria, viruses, fungi, parasites, and prions.
Signal Path
Complete innate immune signaling pathway diagrams by placing missing molecules and identifying activation or inhibition arrows.
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PCR Master Mix
Design primers, run thermocycles, and calculate PCR amplification efficiency using real GFP sequence data.
🎙️
Jeopardy! – PCR Edition
Earn dNTPs by answering PCR questions. Reach the target to start your reaction — Hot Start wagers included!

Clone It!

Level
1
Score
0
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Vector
pUC19
Enzymes:
⊕ Ligation Zone
Cut both plasmids, then drag fragments here
drag fragments here
🗑
Trash
Donor Plasmid(s)
Enzymes:
Activity Log
Time
120s

Clone It!

Score
0
Correct
0
Streak
0
Enzyme
pUC19 — 2686 bp
🔍 560 px
Log
Analysis
Help

Restriction Cloning Tutorial / Challenge mode

1. Select an enzyme and click ✂ Cut Plasmid.

2. Use Show All Sites to see every recognition site.

3. The Analysis tab shows overhangs and ligation predictions.