Clone It!
Cut donor and vector plasmids with restriction enzymes, then ligate the right fragment into the backbone.
1. Vector tab — pick enzymes and cut the vector plasmid
2. Donor tab — pick enzymes and cut the donor plasmid(s)
3. Assemble tab — add fragments, set order & orientation, ligate
Scoring: 100 pts correct · 40 pts reversed · 0 pts wrong combo
Tip: sticky ends must be complementary to ligate!
Select enzyme(s)
Select enzyme(s)
Cut both plasmids first, then add fragments here.
Assembly